[Blueboard] Invitation for the MS Chemistry Thesis Defense of Ms. Shardae Guico

Gina B. Mamauag gmamauag at ateneo.edu
Thu May 5 16:17:18 PHT 2016


​The Department of Chemistry

School of Science and Engineering



Invites you to the MS Thesis Defense of

*Ms. SHARDAE C. GUICO *

*MS Chemistry*



*Title:*  "*ANTI-METASTATIC POTENTIAL OF CACAO, CASSAVA, AND COTTON PLANT
LEAF EXTRACTS VIA INHIBITION OF CANCER CELL MIGRATION*."

*Panelists:*

Olivia Erin M. Buenafe, PhD

Crisanto M. Lopez, PhD

Sonia D. Jacinto, PhD



*Adviser:*

Nina Rosario L. Rojas, PhD

*Date:*  May 10, 2016

*Time:*  4:00 PM

*Venue*: C205, Schmitt Hall



*Thesis Abstract:*

Around 90% of deaths in cancer are due to metastasis—the spread of cancer
cells throughout the body. Matrix metalloproteinase 9 (MMP-9) plays a key
role in this process by degrading components of the extracellular matrix
(ECM) and basement membrane (BM) to facilitate movement. Meanwhile,
medicinal plants are gaining attention as a source of anti-cancer products.
Hence, the objective of this research is to test the potential of medicinal
plant leaf extracts to inhibit the migration of cancer cells, and determine
their effect on MMP-9. Specifically, crude aqueous-ethanolic extracts from
the leaves of Theobroma cacao (cacao), Manihot esculenta (cassava), and
Ceiba pentandra (cotton), were tested and found to inhibit the migration of
LNG4 lung cancer by 30-40% at 500 µg/mL, as well as the migration of HCT116
colon cancer by 50-60% at 500 µg/mL in a wound-healing assay. The
migration-inhibitory activity of these extracts is comparable to that of
anti-metastatic agents, such as MMP-9 inhibitor II and α- mangostin.
Western blot analysis showed that the observed migration-inhibition
involved the intracellular accumulation of the inactive form of MMP-9.
Inside the cell, MMP-9 is not capable of facilitating migration. An MTT
assay showed that at 10–500 µg/mL, the C. pentandra extract had no
cytotoxic side effect on both cell lines while T. cacao and M. esculenta
extracts showed low selective cytotoxicity to HCT116. This work
demonstrates the anti-metastatic potential of medicinal plants in colon and
lung cancer. Keywords: cancer, metastasis, migration, wound-healing assay,
cytotoxicity to HCT116.

This work demonstrates the anti-metastatic potential of medicinal plants in
colon and lung cancer. Keywords: cancer, metastasis, migration,
wound-healing assay, cytotoxicity cytotoxicity to HCT116. This work
demonstrates the anti-metastatic potential of medicinal plants in colon and
lung cancer. Keywords: cancer, metastasis, migration, wound-healing assay,
cytotoxicity to HCT116.




*Gina Mamauag-Buan*

*Chemistry Department*
*Ateneo de Manila University*
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