[Blueboard] Invitation to the MS Chemistry Thesis Defense of Ms. ​MAISIE KIRSTINE ISOBELLE B. CAHAMBING

Gina B. Mamauag gmamauag at ateneo.edu
Wed Nov 19 08:48:50 PHT 2014


​The Department of Chemistry

School of Science and Engineering



Invites you to the MS Chemistry Thesis Defense of

*MS. *
*​​*
*MAISIE KIRSTINE ISOBELLE B. CAHAMBING*


*Title:* DESIGN OF A GENOME-BASED LATERAL FLOW STRIP BIOSENSOR FOR
DETECTION OF WHITE SPOT SYNDROME VIRUS IN SHIRMP


*Panelists:*

1. DR. Leobert Dela Peña

2. Dr. Ronaldo Fabicon

3. Dr. Fabian M. Dayrit



*Adviser*
*​s​*
*:*

1.       Dr. Erwin P. Enriquez

2.       Dr. Nina Rosario A. Rojas



*Date:* 21 November, 2014

*Time:*  10:00 am

*Venue*: C205, Schmitt Hall



*Thesis Abstract:*

*The Philippines is one of the largest producers of the black tiger shrimp
(Penaeus monodon) and the white shrimp (**Litopenaeus vannamei) i**n the
world. However, the shrimp industry still continues to suffer threats of
outbreaks of various pathogens, particularly the white spot syndrome virus
(WSSV), a highly lethal and contagious virus that causes shrimp death.
Immediate detection of the virus is essential in the success of managing
the disease. This research aims to design a biosensor which is
point-of-care, easy-to-use, fast, selective and sensitive for potential use
in field screening detection of the virus. *

*The biosensor is designed using oligonucleotides and gold nanoparticles
(AuNPs) for detection in a competitive binding assay in a lateral flow
strip biosensor (LFSB). Short ssDNA strands with a sequence specific to the
WSSV DNA are conjugated to gold nanoparticles and are allowed to hybridize
with the target DNA. If no target DNA is present, the ssDNA-conjugated
AuNPs hybridize with a complementary DNA probe on the test line of the
nitrocellulose membrane. The LFSB also incorporates a control line wherein
poly-T-conjugated AuNPs hybridize on a poly-A probe on the nitrocellulose
membrane.  The detection limit of the test strips to both target DNA and
spiked crude lysates can reach up to 10-7M. *
*Furthermore, two DNA extraction steps with possible field work
applicability were investigated: DNA extraction using DNAzol®, a non-toxic,
ready-to-use extraction buffer, and a rapid boiling DNA extraction using
SDS and NaOH solution. It is found that optimal volumes of crude lysates
and optimal viewing time to read results are needed to minimize the matrix
effect (shrimp extract) that interfere with the detection of the target DNA
in the LFSB strip.*​

​Thank you.​



*Gina Mamauag-Buan*

*Chemistry Department*
*Ateneo de Manila University*
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