[Blueboard] Invitation to MS Chemistry Thesis Defense of Mona Lisa Lacson

Gina B. Mamauag gmamauag at ateneo.edu
Sat Nov 15 09:53:01 PHT 2014


​The Department of Chemistry

School of Science and Engineering



Invites you to the MS Chemistry Thesis Defense of *MS. MONA LISA B. LACSON*



*Title:* INVESTIGATING THE IN VITRO AND IN VIVO ANGIOGENIC ACTIVITY OF FIVE
PHILIPPINE MEDICINAL PLANTS ASSOCIATED WITH WOUND HEALING PROPERTIES



*Panelists:*

1. Dr. Modesto T. Chua

2. Dr. Merab A. Chan

3. Dr. Fabian M. Dayrit



*Adviser:*

1. Dr. Nina Rosario A. Rojas



*Date:* 17 November, 2014

*Time:* 4:30pm

*Venue*: C205, Schmitt Hall



*Thesis Abstract:*

                Plants have been used since time immemorial to treat many
ailments and speed up the healing process. Plant parts and extracts have
been traditionally used to heal wounds. Angiogenesis is one of the events
associated with wound healing. It is a tightly regulated process of blood
vessel formation and an important target for diseases like coronary
infarction, ischemia and stroke.

                Several in vitro and in vivo methods have been developed to
assess the angiogenic activity of different compounds. These include the
chorio-allantaoic membrane (CAM) assay which uses the egg’s gas exchange
membrane to assess the angiogenic potential of a substance and the tube
formation assay which uses endothelial cells grown in the lab.

                Aqueous ethanolic extracts of five Philippine medicinal
plants associated with wound healing were used in the study. Preliminary
phytochemical screening using spray reagents and toxicity test using brine
shrimp (Artemia salina) nauplii was done. 10% of the computed LC50 value
was used to screen the angiogenic potential of the plant extracts using
human umbilical vein endothelial cells (HUVECs) in an in vitro tube
formation assay and in vivo chorio-allantoic membrane (CAM) assay. Phorbol
myristate (PMA) a known pro-angiogenic compound was used as positive
control.
                Initial phytochemical screening showed that the crude
ethanolic extracts of the leaves of the five plants contain flavonoids,
steroids, phenols, saponins, alkaloids, coumarins, anthranoids,
anthraquinones, sugars and essential oils. Brine shrimp lethality assay
revealed that the plants used were not toxic to Artemia salina nauplii at
1000 µg/mL. In vitro tube formation assay revealed the crude ethanolic
extracts of the leaves of M. indica showed the greatest angiogenic
activity, closely followed by C. pubescens, T. catappa, A. barbadensis and
C. odorata. The effect of the crude ethanolic extracts of the plants on
blood vessel formation in the in vivo CAM model showed A. barbadensis with
the highest angiogenic activity, closely followed C. odorata, C. pubescens,
M. indica and T. catappa.​

​Thank you very much!​


*Gina Mamauag-Buan*

*Chemistry Department*
*Ateneo de Manila University*
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