[Blueboard] "Systematic analysis of E. Coli genes/ORFs involved in biofilm formation"

falcantara at ateneo.edu falcantara at ateneo.edu
Tue Feb 24 08:50:31 PHT 2004


The Biology Department cordially invites everyone to attend a seminar to
be presented by Dr. Elizabeth L. Tenorio, Ph in Biology (Kobe
University,
Japan).  The talk will be on March 10, 2004 at 3:30-4:30 pm SEC Lecture
Hall 2. 
     The title of the talk is 

"Systematic analysis of E. coli genes/ORFs involved in biofilm formation"

Tenorio E a, Kitakawa Mb, Isono Sb, Baba Tc, and Mori Md,e, and Isono
Ke,f.
aGraduate School of Science and Technology, Kobe University, bDepartment
of 
Biology, Kobe University, cKeio University, dNara Advanced Institute of 
Science and Technology, eCREST Japan, and efNITE, Japan

ABSTRACT
Bacteria such as E. coli have been mainly considered as single cellular 
organisms. However, they rarely exist as isolated cells in nature. Recent

studies have indicated the presence of cooperative behavior that can be 
judged by observing patterns of gene expression, spatial patterns in
colony 
morphology, and biofilm growth. Bacterial biofilm formation follows a 
developmental plan influenced by environmental signals and requires a
higher 
level of organization amongst cells. Different functional groups of genes

such as those required for the synthesis of flagella, outer-membrane 
adhesins and exopolysaccharides are generally involved. However, little
is

known about the regulatory process for the transition of bacterial cells 
from planktonic to biofilm stage. Previously a complete set of E. coli
genes 
cloned into an expression vector was used to identify various genes that 
affect biofilm formation upon over-expression. To expand the study, a 
collection of deletion mutants constructed in BW25113 strain using
"one-step 
gene deletion" method was screened for biofilm phenotype. The results 
indicated the requirement of various genes for energy production, carbon 
regulation, protein degradation and outer-membrane structures. Several 
two-component systems were found to link flagella and curli production, 
two-component signaling, quorum sensing and biofilm development. Further 
studies are underway to understand the process of biofilm development.




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